Sa. Luther et al., Coexpression of the chemokines ELC and SLC by T zone stromal cells and deletion of the ELC gene in the plt/plt mouse, P NAS US, 97(23), 2000, pp. 12694-12699
Citations number
30
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
The spontaneous mutant mouse strain, plt/plt, lacks the secondary lymphoid
organ chemokine (SLC)-ser gene and has disrupted trafficking of T cells and
dendritic cells (DCs) to lymphoid tissues. We demonstrate here that the ge
ne for the related chemokine, Epstein-Barr virus-induced molecule-1 ligand
chemokine (ELC), is also deleted in this immunodeficient mouse strain. Usin
g a combination of approaches, including bone marrow reconstitution and dou
ble in situ hybridization, we show in wild-type mice that ELC is expressed
by T zone stromal cells that also make SLC. Smaller amounts of ELC are made
by DCs, predominantly of the CD8(+) phenotype. We propose that ELC- and SL
C-expressing T zone stromal cells play a central role in bringing naive T c
ells and DCs together for the initiation of immune responses.