Analysis of near-isogenic lines of common wheat carrying the dominant alleles of Bg, Hg, and Rg1 genes using microsatellite and protein markers

To determine tight linkage between morphological and molecular markers of t he first homeologous group of chromosomes of common wheat, microsatellite a nalysis of six near-isogenic lines with marker dominant alleles controlling black color (Bg; 1AS) and hairy glume (Hg; 1AS) and two lines bearing the dominant alleles of the gene for red glume color (Rgl; 1BS) was conducted. The component composition of gliadins in these lines was studied. Tight lin kage of Bg, Hg, and Gli-A1 genes with a microsatellite marker Xgwm136 (1AS) and of Rg1 and Gli-B1 genes with markers Xgwm33 and Xgwm550 (1BS) was show n. Based on the results obtained and literature data, the most probable ord er of morphological and molecular markers on chromosomes of common wheat wa s determined. On chromosome 1AS, from the centromere to the telomere, the m arkers are located as follows: Xgwm136-Gli-A1-Bg/Hg; on chromosome 1BS, in the same direction: Xgwm33-Gli-B1-Rg1-Xgwm550.