RAI, one candidate gene associated with differentiation of human lung adenocarcinoma cells

From all-trans retinoic acid (ATRA)-treated human lung adenocarcinoma GLC-8 2 cells and control, subtractive cDNA library has been constructed using su btractive hybridization technique in our laboratory. The screening of the c DNA subtractive library resulted in identification of a clone containing cD NA fragment of one ATRA-induced gene (RAI) in GLC-82 cells. The positive cl one with full-length cDNA of RAI was identified by screening fetal brain cD NA library using colony hybridization technique, and then sequenced. RT-PCR results showed that RAI was expressed in many different human fetal tissue s. These results suggest that RAI may be involved in cell differentiation a nd play an important role in vital activities of cells.