Physicochemical properties and inclusion behavior of panosyl-alpha-cyclodextrin

Panosyl-alpha -cyclodextrin was synthesized from alpha -cyclodextrin and pa nose through the revel-se action of Bacillus acidopullulyticus pullulanase and purified using high-performance liquid chromatography on ODS and amino columns. The physicochemical and biological properties and solubilization c apability of panosyl-alpha -cyclodextrin were compared with those of conven tional alpha -cyclodextrin and branched alpha -cyclodextrins. The hemolytic activity of panosyl-alpha -cyclodextrin was lower than those of alpha -cyc lodextrin and glucosyl-alpha -cyclodextrin. Panosyl-alpha -cyclodextrin was found to be slightly unstable in an acid solution, with stability similar to that of maltosyl-alpha -cyclodextrin. The solubilization of panosyl-alph a -cyclodextrin for drugs slightly soluble or insoluble in water was greate r than that of alpha -cyclodextrin but the same as that of conventional bra nched alpha -cyclodextrin. The suppressive effects on the photolysis of mec obalamine in the presence of alpha -cyclodextrins were in the following ord er: panosyl-alpha -cyclodextrin > maltosyl-alpha -cyclodextrin > glucosyl-a lpha -cyclodexatrin > alpha -cyclodextrin.