Biolistic (R) transformation of cacao [Theobroma cacao (L.)]

Authors
Perry, MD Power, JB Lowe, KC Davey, MR
Citation
Md. Perry et al., Biolistic (R) transformation of cacao [Theobroma cacao (L.)], TROP AGR, 77(1), 2000, pp. 64-66
Citations number
12
Categorie Soggetti
Agriculture/Agronomy
Journal title
TROPICAL AGRICULTURE
ISSN journal
00413216 → ACNP
Volume
77
Issue
1
Year of publication
2000
Pages
64 - 66
Database
ISI
SICI code
0041-3216(200001)77:1<64:B(TOC[>2.0.ZU;2-Q
Abstract
Callus of cacao [Theobroma cacao (L.)] were transformed by particle bombard ment (Biolistics(R)). Four-week-old floral-derived calli were bombarded wit h plasmid containing the beta -glucuronidase (gus) gene, driven by a CaMV 3 5S promoter. Histochemical analysis of gus gene expression was used to opti mize the parameters for deoxyribonucleic acid (DNA) delivery. More than 270 histochemically positive (blue staining) transformation events were observ ed with staminode-derived calli bombarded at 1550 psi.