Different effects of di- and triphenyltin compounds on lipid bilayer dithionite permeabilization

Phenyltins are chemicals widely used in industry, hence their occurrence in the human environment is frequent and widespread. Such compounds include h ydrophobic phenyl rings bonded to positively charged tin. This molecular st ructure makes them capable of adsorbing onto and penetrating through biolog ical membranes, hence they are potentially hazardous. Two such compounds, d iphenyltin and triphenyltin, show different steric constraints when interac ting with the lipid bilayer. It has been demonstrated that these compounds are positioned at different locations within model lipid bilayers, causing dissimilarity in their ability to affect membrane properties. In this paper we present a study regarding the ability of these two phenyltins to facili tate the transport of S2O4-2 ions across the lipid bilayer, evaluated by a fluorescence quenching assay. In concentration range of up-to 60 muM those compounds do not affect lipid bilayer topology when evaluated by vesicle si ze distribution. Both phenyltins Facilitate the transfer of S2O4-2 across t he model Lipid bilayer, but the dependence of dithionite transport on pheny ltin concentration is different for both. In principle, above 20 muM triphe nyltin is more efficient in transferring ions across the lipid bilayer than diphenyltin.