Iron(II)-mimosine catalyzed cleavage of DNA

Authors
Mikhailov, I Ninova, P Russev, G Anachkova, B
Citation
I. Mikhailov et al., Iron(II)-mimosine catalyzed cleavage of DNA, Z NATURFO C, 55(9-10), 2000, pp. 849-851
Citations number
12
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
ISSN journal
09395075 → ACNP
Volume
55
Issue
9-10
Year of publication
2000
Pages
849 - 851
Database
ISI
SICI code
0939-5075(200009/10)55:9-10<849:ICCOD>2.0.ZU;2-6
Abstract
Supercoiled plasmid DNA was treated in vitro with H2O2, DTT and either Fe ( II), Fe (II)-EDTA or Fe (II)-mimosine. The rate of DNA break formation was followed by the conversion of the supercoiled form into relaxed-circular an d linear forms. In the concentration interval of 0-4 muM Fe (II), Fe (II)-E DTA slowed-down the formation of DNA breaks, while Fe (II)-mimosine enhance d the rate of break formation up to several times. A conclusion is drawn th at this enhancement is due to the increased affinity of the Fe (II)-mimosin e complex to DNA.