Sn. Wai et al., A comparison of solid and liquid media for resuscitation of starvation- and low-temperature-induced nonculturable cells of Aeromonas hydrophila, ARCH MICROB, 173(4), 2000, pp. 307-310
Like many other gram-negative bacteria, starved cells of Aeromonas hydrophi
la can be induced into a viable but nonculturable (VBNC) state by incubatio
n at low temperature, as shown here by using various bacterial enumeration
methods. Starved A. hydrophila strain HR7 cells at 4 degreesC reached the n
onculturable stage in about 45 days. The cells were resuscitated by either
a solid medium resuscitation method, using solid agar amended with H2O2-deg
rading agents, catalase or sodium pyruvate, or a liquid medium resuscitatio
n method, by incubating nonculturable cells in liquid media containing thes
e compounds before spreading onto plates. The liquid medium resuscitation m
ethod using catalase resulted in nearly complete recovery of nonculturable
cells.