Host-guest complex formation in cyclotrikis-(1 -> 6)[alpha-D-glucopyranosyl-(1 -> 4)-beta-D-glucopyranosyl]

The possibility that cyclotrikis-(1 --> 6)-[alpha -D-glucopyranosyl-(1 --> 4)-beta -D-glucopyranosyl] (CGM6) forms inclusion complexes, like cycloamyl oses (cyclodextrins), was investigated by means of electrospray mass spectr ometry and fluorescence spectroscopy. The complexing ability of both 1-anil inonaphthalene-8-sulfonate (ANS) and 2-p-toluidinylnaphthalene-6-sulfonate (TNS), which were already used with cyclodextrins, was investigated. The fo rmer showed very little or no tendency to be complexed by CGMG, while the l atter produced detectable adducts with CGMG. Fixed 90 degrees angle light s cattering experiments supported the findings obtained by molecular modellin g calculations, which indicated a polar character for the CGMG internal cav ity. CGM6-TNS complexes were probably formed throughout interaction of the polar regions of the two molecules. (C) 2000 Elsevier Science Ltd. All righ ts reserved.