Ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter

Here, we show that the IFN-beta enhanceosome activates transcription by dir ecting the ordered recruitment of chromatin modifying and general transcrip tion factors to the IFN-beta promoter. The enhanceosome is assembled in the nucleosome-free enhancer region of the IFN-beta gene, leading to the modif ication and remodeling of a strategically positioned nucleosome that masks the TATA box and the start site of transcription. Initially, the GCN5 compl ex is recruited, which acetylates the nucleosome, and this is followed by r ecruitment of the CBP-PoIII holoenzyme complex. Nucleosome acetylation in t urn facilitates SWI/SNF recruitment by CBP, resulting in chromatin remodeli ng. This program of recruitment culminates in the binding of TFIID to the p romoter and the activation of transcription.