Placental alkaline phosphatase, insulin, and adenine nucleotides or adenosine synergistically promote long-term survival of serum-starved mouse embryo and human fetus fibroblasts
Qb. She et al., Placental alkaline phosphatase, insulin, and adenine nucleotides or adenosine synergistically promote long-term survival of serum-starved mouse embryo and human fetus fibroblasts, CELL SIGNAL, 12(9-10), 2000, pp. 659-665
Earlier we showed that in serum-starved fibroblasts placental alkaline phos
phatase (PALP) can exert growth factor-like effects. Here we report that in
mouse embryo (NIH 3T3) and human fetus (HTB-157) fibroblasts, PALP (200 nM
) alone provided full protection against serum starvation-induced cell deat
h for 5 days. After 12 days, substantial effects of PALP on cell survival r
equired the copresence of insulin (500 nM) and ATP or adenosine (100 muM).
In serum-starved NIH 3T3 cells, PALP induced activating phosphorylation of
p42/p44 mitogen-activated protein (MAP) kinases; insulin, but not ATP, had
small additional effects. PALP also stimulated the expression of various cy
clins; ATP both prolonged and enhanced PALP-induced expression of cyclins A
and E. Finally, ATP/adenosine enhanced activation of Akt kinase by insulin
. The results suggest that PALP may be a regulator of growth and remodeling
of fetal tissues during the second and third trimester of pregnancy when i
t is expressed. (C) 2000 Elsevier Science Inc. All rights reserved.