Solvent extraction of zein from dry-milled corn

Batch extraction of zein from dry-milled whole corn with ethanol was optimu m with 70% ethanol in water, an extraction time of 30-40 min, and temperatu re of 50 degreesC. High yields (60% of the zein in corn) and high zein cont ents in the extracted solids (50%) were obtained at a solvent-to-solids rat io of 8 mt of 70% ethanol/g of corn. However, zein concentration in the ext ract was higher at lower ratios. Multiple extraction of the same corn with fresh ethanol resulted in a yield of 85% after four extractions, whereas mu ltiple extractions of fresh corn with the same ethanol resulted in high (15 g/L) zein concentration in the extract. Optimum conditions for batch extra ction of zein were 45 degreesC, with 68% ethanol at a solvent-to-solids rat io of 7.8 mL/g for an extraction time of 55 min. Column extractions were al so best at 50 degreesC and 70% ethanol; a solvent ratio of 1 mL/g resulted in high zein concentrations in the extract (17 g/L) but yields were low (20 %).