Determination of gatifloxacin in human serum and urine by HPLC

Authors
Borner, K Hartwig, H Lode, H
Citation
K. Borner et al., Determination of gatifloxacin in human serum and urine by HPLC, CHROMATOGR, 52, 2000, pp. S105-S107
Citations number
7
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
52
Year of publication
2000
Supplement
S
Pages
S105 - S107
Database
ISI
SICI code
0009-5893(2000)52:<S105:DOGIHS>2.0.ZU;2-P
Abstract
A sensitive and specific HPLC method for gatifloxacin applicable to serum a nd urine of volunteers was developed. The method can be operated unattended in over-night runs. it uses reversed-phase HPLC with fluorimetric detectio n. Trovafloxacin serves as internal standard. With a detection limit of 0.0 3 mg L-1 the method was sensitive enough to measure serum concentrations of gatifloxacin in healthy volunteers for 48 hours after a single oral dose o f 400 mg. There was excellent agreement with results obtained by a microbio logical assay.