T. Yoshida et al., Calculation of amino acid hydrophilicity indices for retention of peptideson amide, diol and silica columns in normal-phase liquid chromatography, CHROMATOGR, 52(7-8), 2000, pp. 418-424
A sei of hydrophilicity parameters in a normal-phase liquid chromatography
of peptides is presented in order to clarify the contribution of individual
amino acid residues to peptide retention and to predict retention times. T
he retention of 100 peptides was studied using normal-phase liquid chromato
graphy on amide, diol and silica columns. An acetonitrile-water mixed solut
ion containing 0.2% trifluoroacetic acid +0.2% triethylamine was used as th
e mobile phase in a linear gradient elution system. The contribution of eac
h residue upon retention was calculated by linear multiple regression analy
sis. This paper described the contribution valves as "hydrophilicity retent
ion coefficients". Using these hydrophilicity retention coefficients, reten
tion times could be predicted for peptides of known amino acid content and
sequence A set of hydrophilicity retention coefficients on each column was
successfully explained by contributions to the degree of retention.