Protective effects of calcium against chronic waterborne cadmium exposure to juvenile rainbow trout

Juvenile rainbow trout (Oncorhynchus mykiss [Walbaum]) on 1% daily ration w ere exposed to 0 (control) or 2 mug of cadmium as Cd(NO3)2 . 4H(2)O per lit er added to four different calcium (Ca) concentrations: 260 (background), 4 70 (low), 770 (medium), or 1200 (high) muM of Ca added as Ca(NO3)(2). 4H(2) O in synthetic soft water for 30 d. Mortality was highest (similar to 80%) in the background + Cd treatment. Approximately 40% mortality was observed in the low + Cd exposure; mortality was 10% or less for all other treatment s. No growth effects were seen for any of the exposures. Kidneys accumulate d the greatest concentration of Cd during the 30 d, followed by gills and l ivers. Accumulation of Cd in gills, kidney, and liver decreased at higher w ater Ca concentrations. No differences in whole-body or plasma Ca concentra tions were found. Swimming performance was impaired in the low + Cd-exposed fish. Influx of Ca2+ into whole bodies decreased as water Ca concentration s increased; influx of Ca2+ into background + Cd-treated fish was significa ntly reduced compared to that in control fish. Experiments that measured up take of new Cd into gills showed that the affinity of gills for Cd (KCd-gil l) and the number of-binding sites for Cd decreased as water Ca concentrati ons increased. Acute accumulation of new Cd into gills and number of gill C d-binding sites increased with chronic Cd exposure, whereas the affinity of gills for Cd decreased with chronic Cd exposure. Longer-term gillbinding ( 72 h) showed reduced uptake of new Cd at higher water Ca levels and increas ed uptake with chronic Cd exposure. Complications were found in applying th e biotic ligand model to fish that were chronically exposed to Cd because o f discrepancies in the maximum number of gill Cd-binding sites among differ ent studies.