A. Bouloc et al., Triggering CD101 molecule on human cutaneous dendritic cells inhibits T cell proliferation via IL-10 production, EUR J IMMUN, 30(11), 2000, pp. 3132-3139
Since the CD101 molecule is expressed on a major subpopulation of HLA-DR+,
CD1a(+), CD1c(+) cutaneous dendritic cells (DC), we studied the functional
role of CD101 on cutaneous DC. Anti-CD101 monoclonal antibody (mAb) inhibit
ed the proliferation of T cells induced by cutaneous DC. There was a synerg
istic inhibition between anti-CD101 mAb and anti-CD86/ anti-CD80 mAb. Anti-
CD101 mAb exerted its inhibitory effect when binding to the CD101 expressed
on cutaneous DC. No positive role of CD101 putative ligand expressed by T
cells in T cell proliferation was demonstrated, as T cells proliferated in
response to soluble anti-CD3 mAb in the presence of CD86-transfected cells
but not in the presence of CD101-transfected cells. Of major significance i
s the fact that IL-10 was produced by cutaneous DC after CD101 triggering w
ith anti-CD101 mAb, while IL-10 secretion was up-regulated in mixed cutaneo
us DC-T cell cultures after CD101 triggering. Furthermore, IL-10-neutralizi
ng mAb could reverse the inhibition induced by anti-CD101 mAb. Our results
demonstrate that the CD101 triggering on cutaneous DC inhibits T cell proli
feration via IL-10 production, suggesting an important regulatory role play
ed by the CD101 molecule on DC during T cell activation.