An NMR-based metabonomic approach to investigate the biochemical consequences of genetic strain differences: application to the C57BL10J and Alpk : ApfCD mouse
Cl. Gavaghan et al., An NMR-based metabonomic approach to investigate the biochemical consequences of genetic strain differences: application to the C57BL10J and Alpk : ApfCD mouse, FEBS LETTER, 484(3), 2000, pp. 169-174
As the human genome sequencing projects near completion, there is an active
search for technologies that can provide insights into the genetic basis f
or physiological variation and interpreting gene expression in terms of phe
notype at the whole organism level in order to understand the pathophysiolo
gy of disease. We present a novel metabonomic approach to the investigation
of genetic influences on metabolic balance and metabolite excretion patter
ns in two phenotypically normal mouse models (C57BL10J and Alpk:ApfCD). Che
mometric techniques were applied to optimise recovery of biochemical inform
ation from complex H-1 NMR urine spectra and to determine metabolic biomark
er differences between the two strains. Differences were observed in tricar
boxylic acid cycle intermediates and methylamine pathway activity. We sugge
st here a new 'metabotype' concept, which will be of value in relating quan
titative physiological and biochemical data to both phenotypic and genetic
variation in animals and man, (C) 2000 Federation of European Biochemical S
ocieties. Published by Elsevier Science B,V, All rights reserved.