Molecular cloning of an Atlantic salmon nucleoside diphosphate kinase cDNAand its pattern of expression during embryogenesis

Citation
M. Murphy et al., Molecular cloning of an Atlantic salmon nucleoside diphosphate kinase cDNAand its pattern of expression during embryogenesis, GENE, 257(1), 2000, pp. 139-148
Citations number
41
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENE
ISSN journal
03781119 → ACNP
Volume
257
Issue
1
Year of publication
2000
Pages
139 - 148
Database
ISI
SICI code
0378-1119(20001017)257:1<139:MCOAAS>2.0.ZU;2-U
Abstract
To gain insight into the process of development in Atlantic salmon (Salmo s alar), we sought to identify genes that were differentially expressed at ga strulation. A polymerase chain reaction-based differential screening strate gy allowed for the isolation of an Atlantic salmon nucleoside diphosphate k inase cDNA (nm23). Structural characterisation showed a high degree of homo logy with a large number of previously isolated nucleoside diphosphate kina ses (NM23s), both prokaryote and eukaryote, though it represents the first teleost nucleoside diphosphate kinase identified. Highest similarities were found with the type 1 and type 2 NM23 isoforms of mammals. Phylogenetic an alysis indicates that the duplication event that gave rise to these isoform s occurred after the splitting of tetrapods and fish, suggesting that the s almon NM23 represents a more ancestral isoform. The position of the salmon sequence on the phylogenetic tree indicates that the salmon genome is expec ted to have at least three copies of genes from the nm23 gene family. North ern blot analysis showed a single transcript of approximately 0.7 kb in bot h embryonic and adult tissues. Examination of the temporal pattern of expre ssion of salmon nucleoside diphosphate kinase during embryonic development revealed that this gene is first expressed at the time of gastrulation. Nuc leoside diphosphate kinases are thought to have a vital role in regulatory processes such as signal transduction, proliferation and differentiation. T aken together, these results suggest that nucleoside diphosphate kinases ha ve an important role to play in early embryogenic development in vertebrate s. (C) 2000 Elsevier Science B.V. All rights reserved.