Omeprazole and regulation of cytokine profile in Helicobacter pylori-infected patients with duodenal ulcer disease

Background/Aims: To estimate the cytokine profile in Helicobacter pylori-po sitive patients with duodenal ulcers treated with omeprazole. Methodology: The subjects were 22 patients with endoscopically defined acti ve duodenal ulcers and H. pylori infection treated with omeprazole for 3 mo nths after initial 1-week triple therapy. Peripheral blood CD3(+) CD4(+) an d CD8(+) T lymphocytes, percentage of HLA-DR+ peripheral blood lymphocytes, and cytokine levels (GM-CSF, IL6 and IL-2) were measured in the supernatan ts from PHA-cultured peripheral blood lymphocytes at baseline conditions an d after 3-months' omeprazole treatment. Results: The H. pylori eradication triple regimen cured H. pylori infection in 86.4% of our patients. Peripheral blood CD3(+) CD4(+) and CD8(+) T lymp hocytes were not affected by omeprazole treatment (P>0.05), whereas the per centage of HLA-DR+ peripheral blood lymphocytes increased significantly pos t-treatment (P<0.05). Al cytokine levels measured in the supernatants showe d a significant decrease after treatment (P<0.001). Conclusions: The significant decrease in the two pro-inflammatory cytokines (GM-CSF, IL-6) and the major inflammatory cytokine IL-2, implies that the potential cytokine synthesis of the Th1 pattern from peripheral blood lymph ocytes is highly suppressed after treatment. This effect on cytokine regula tion seems to be a result of the positive H. pylori infection outcome at th e immunological level, which leads to "normalization" of cytokine productio n.