Protein synthesis in H9c2 heart-derived myocytes responds biphasically to a
rginine vasopressin (1 muM). An initial 50% inhibition attributable to Ca2 mobilization from the sarcoplasmic/endoplasmic reticulum is followed by a
recovery that subsequently converts to a 1.5-fold stimulation. This study w
as undertaken to ascertain whether vasopressin programs H9c2 cells to under
go hypertrophy or to proliferate and whether early translational inhibition
is required for programming. Translational suppression was observed only a
t vasopressin concentrations (>1 nM) causing extensive (> 50%) depletion of
Ca2+ stores and was diminished at supraphysiologic extracellular Ca2+ conc
entrations. Stimulation of protein synthesis, by contrast, was unaffected b
y changes in extracellular Ca2+ depended on gene transcription, was suppres
sed by a protein kinase C pseudosubstrate sequence (peptide 19-27), and was
observed at phl vasopressin concentrations. Activation of MAP kinases, pho
sphoinositide 3-kinase, calcineurin, S6 kinase, ol elF4 could not be implic
ated in the stimulation, which persisted for 24 h. Vasopressin-treated H9c2
cells underwent hypertrophy by standard criteria. Cellular protein accumul
ation occurred at pM hormone concentrations, was blocked by peptide 19-27,
was observed regardless of retinoic acid pretreatment to prevent myogenic t
ransdifferentiation, and preceded full repletion of Ca2+ stores. It is prop
osed that H9c2 cells, which possess all basic features of V1-vasoplessin re
ceptor signaling, provide a convenient model for investigating vasopressin-
induced myocyte hypertrophy. Early translational suppression is not needed
for vasopressin-induced H9c2 myocyte hypertrophy whereas activation of prot
ein kinase C appears essential. (C) 2000 Elsevier Science Ltd. All rights r
eserved.