Various seminal quality tests, including sperm morphology, sperm motil
ity, cervical mucus penetration, zona pellucida penetration, osmotic s
welling, supravital staining, fluorescent staining, and flow cytometri
c analyses are described in relation to their ability to identify pote
ntial fertility in animals. The new automated semen analyses systems r
eviewed in this article tend to provide more reliable information, but
may not be cost-effective. The determination of true fertility, which
can be assessed only by the number of Live offspring, will continue t
o be required no matter how sophisticated the seminal quality methodol
ogy becomes.