Effects of BisGMA and TEGDMA on proliferation, migration, and tenascin expression of human fibroblasts and keratinocytes

Previous studies have documented a marked cytotoxic potency of BisGMA and T EGDMA. The purpose of this investigation was to determine if these substanc es also affect proliferation, migration, and tenascin expression of primary human gingival fibroblasts (HGF) and immortalized human keratinocytes (HaC aT), These parameters play an important role in healing wounds, HGF and HaC aT cultures were incubated with TEGDMA and BisGMA, Cell proliferation (BrdU -assay) and migration (Boyden method) were determined 24 h after incubation , Tenascin expression was investigated four and seven days after treatment, Results were statistically evaluated by ANOVA using the Wilcoxon-Mann-Whit ney test (p < 0.05), Proliferation of both cell types was significantly inh ibited at concentrations <greater than or equal to> 0.25 mM (TEGDMA) or gre ater than or equal to 0.01 mM (BisGMA). Migration of KaCaT was significantl y increased after incubation with BisGMA for 24 h. TEGDMA did not alter mig ration of HGF and HaCaT. In addition, TEGDMA had no effect on tenascin expr ession of both cell cultures. After 4 days of incubation, BisGMA (at a conc entration of 0.01 mM) significantly reduced tenascin production of HaCaT cu ltures related to cell number. However, 7 days after treatment, BisGMA sign ificantly increased tenascin expression of HGF and HaCaT cultures. Altogeth er, our results indicate that BisGMA can affect migration of keratinocytes and alters the expression of the extracellular matrix component tenascin, T hus, BisGMA may significantly influence the healing of injured oral tissues , (C) 2000 John Wiley & Sons, Inc.