C. Theilig et al., Effects of BisGMA and TEGDMA on proliferation, migration, and tenascin expression of human fibroblasts and keratinocytes, J BIOMED MR, 53(6), 2000, pp. 632-639
Previous studies have documented a marked cytotoxic potency of BisGMA and T
EGDMA. The purpose of this investigation was to determine if these substanc
es also affect proliferation, migration, and tenascin expression of primary
human gingival fibroblasts (HGF) and immortalized human keratinocytes (HaC
aT), These parameters play an important role in healing wounds, HGF and HaC
aT cultures were incubated with TEGDMA and BisGMA, Cell proliferation (BrdU
-assay) and migration (Boyden method) were determined 24 h after incubation
, Tenascin expression was investigated four and seven days after treatment,
Results were statistically evaluated by ANOVA using the Wilcoxon-Mann-Whit
ney test (p < 0.05), Proliferation of both cell types was significantly inh
ibited at concentrations <greater than or equal to> 0.25 mM (TEGDMA) or gre
ater than or equal to 0.01 mM (BisGMA). Migration of KaCaT was significantl
y increased after incubation with BisGMA for 24 h. TEGDMA did not alter mig
ration of HGF and HaCaT. In addition, TEGDMA had no effect on tenascin expr
ession of both cell cultures. After 4 days of incubation, BisGMA (at a conc
entration of 0.01 mM) significantly reduced tenascin production of HaCaT cu
ltures related to cell number. However, 7 days after treatment, BisGMA sign
ificantly increased tenascin expression of HGF and HaCaT cultures. Altogeth
er, our results indicate that BisGMA can affect migration of keratinocytes
and alters the expression of the extracellular matrix component tenascin, T
hus, BisGMA may significantly influence the healing of injured oral tissues
, (C) 2000 John Wiley & Sons, Inc.