Production of cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) by Rhodococcus sp MA 7249

Authors
Chartrain, M Ikemoto, N Taylor, C Stahl, S Sandford, V Gbewonyo, K Chirdo, C Maxwell, C Osoria, J Buckland, B Greasham, R
Citation
M. Chartrain et al., Production of cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene (toluene cis glycol) by Rhodococcus sp MA 7249, J BIOSCI BI, 90(3), 2000, pp. 321-327
Citations number
30
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
90
Issue
3
Year of publication
2000
Pages
321 - 327
Database
ISI
SICI code
1389-1723(200009)90:3<321:POC(CG>2.0.ZU;2-P
Abstract
An attractive method for producing cis-1,2-dihydroxy-3-methylcyclohexa-3,5- diene (toluene cis glycol) was developed employing a cis dihydrodiol dehydr ogenase "deficient" strain of Rhodococcus (MA 7249). The toluene cis glycol produced was found to have optical rotations of [alpha](D)(25) = + 25.8 (c 0.45, CH3OH) and + 72.8 (c 0.42, CHCl3) which indicated an absolute config uration of (LT,2R) when compared with previously published values. When cul tivated in laboratory fermenter in the presence of toluene vapors, MA 7249 reached a toluene cis glycol concentration up to 18 g/l in 110 h. Culture M A 7249 also accumulated cis (1S,2R) dihydrodiols from dihydronaphthalene, b iphenyl, chlorobenzene, and styrene.