Celloidin mounting (embedding without infiltration) - a new, simple and reliable method for producing serial sections of high thickness through complete human brains and its application to stereological and immunohistochemical investigations

Authors
Heinsen, H Arzberger, T Schmitz, C
Citation
H. Heinsen et al., Celloidin mounting (embedding without infiltration) - a new, simple and reliable method for producing serial sections of high thickness through complete human brains and its application to stereological and immunohistochemical investigations, J CHEM NEUR, 20(1), 2000, pp. 49-59
Citations number
34
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF CHEMICAL NEUROANATOMY
ISSN journal
08910618 → ACNP
Volume
20
Issue
1
Year of publication
2000
Pages
49 - 59
Database
ISI
SICI code
0891-0618(200010)20:1<49:CM(WI->2.0.ZU;2-K
Abstract
Celloidin mounting (embedding without infiltration) of the human central ne rvous system (CNS) proved to be superior to gelatin embedding for the produ ction of serial sections ranging in thickness from 220 to 500 mum. After ga llocyanin-staining, a comprehensive neuroanatomical as well as neuropatholo gical survey of the human brain is possible, including diagnosis of Alzheim er's disease. Details of a fractionator analysis of the total striatal neur on number are described and the possible quantitative analysis of parallel immunohistochemically stained sections is discussed. (C) 2000 Elsevier Scie nce B.V. All rights reserved.