Northeast Italy population data using multiplex PCR (HUMCD4, HUMTH01, HUMTPOX, and HUMCSF1P0) loci

Allele and genotype frequencies for four short tandem repeat (STR) loci (HU MCD4, HUMTH01, HUMTPOX, and HUMCSF1P0) were determined in 100 unrelated ind ividuals from Veneto (Northeast Italy). After a multiplex polymerase chain reaction (PCR)-amplification, semi-automatic DNA profiling was performed us ing an A.L.F.express DNA Sequencer. Conditions were optimized for the PCR c o-amplification of these four STR loci and the quadruplex PCR was performed on various forensic DNA samples such as whole blood, blood-stains, teeth, and saliva from Caucasians living in the Northeast Italy. The distribution of the genotype frequencies showed no significant deviation from Hardy-Wein berg expectations in the sampled population. The combined Power of Discrimi nation (PD) of the quadruplex was 0.9999.