M. Schwab et al., Effects of betamethasone administration to the fetal sheep in late gestation on fetal cerebral blood flow, J PHYSL LON, 528(3), 2000, pp. 619-632
1. Glucocorticoid administration to women at risk of preterm delivery to ac
celerate fetal lung maturation has become standard practice. Antenatal gluc
ocorticoids decrease the incidence of intraventricular haemorrhage as well
as accelerating fetal lung maturation. Little is known regarding side effec
ts on fetal cerebral function. Cortisol and synthetic glucocorticoids such
as betamethasone increase fetal blood pressure and femoral vascular resista
nce in sheep.
2. We determined the effects of antenatal glucocorticoid administration on
cerebral blood flow (CBF) in fetal sheep. Vehicle (n = 8) or betamethasone
(n = 8) was infused over 48 h via the jugular vein of chronically instrumen
ted fetal sheep at 128 days gestation (term 146 days). The betamethasone in
fusion rate was that previously shown to produce fetal plasma betamethasone
concentrations similar to human umbilical vein concentrations during anten
atal glucocorticoid therapy.
3. Regional CBF was measured in 10 brain regions, using coloured microspher
es, before and 24 and 48 h after onset of treatment, and during hypercapnic
challenges performed before and 48 h after onset of betamethasone exposure
. Betamethasone exposure decreased CBF in all brain regions measured except
the hippocampus after 24 h of infusion (P < 0.05). The CBF decrease was mo
st pronounced in the thalamus and hindbrain (45-50% decrease) and least pro
nounced in the cortical regions (35-40% decrease). It was mediated by an in
crease in cerebral vascular resistance (CVR, P < 0.05) and led to a decreas
e in oxygen delivery to subcortical and hindbrain structures of 30-40%, to
8.6 +/- 1.1 ml (100 g)(-1) min(-1), and 40-45%, to 11.0 +/- 1.6 ml 100 g(-1
) min(-1) respectively (P < 0.05).
4. After 48 h of betamethasone treatment, the reduction in CBF was diminish
ed to about 25-30%, but was still significant in comparison to vehicle-trea
ted fetuses in all brain regions except three of the five measured cortical
regions (P < 0.05). CVR and oxygen delivery were unchanged in comparison t
o values at 24 h of treatment. The CBF increase in response to hypercapnia
was diminished (P < 0.05).
5. These observations demonstrate for the first time that glucocorticoids e
xert major vasoconstrictor effects on fetal CBF This mechanism may protect
the fetus against intraventricular haemorrhage both at rest and when the fe
tus is challenged. Betamethasone exposure decreased the hypercapnia-induced
increase in CBF (P < 0.05) due to decreased cerebral vasodilatation (P < 0
.05).