The oxidation reaction of squalene is investigated using a bubble column re
actor under the various conditions. For oxidation, two stages, which are an
induction period with a slow increase in hydroperoxide and an accelerated
period with rapid increase, are observed. The peroxide concentration increa
ses with the progress of the oxidation and reaches a maximum value in the l
ast part of the accelerated period, and then decreases again due to the dec
omposition of peroxide. The peroxide produced is analyzed by a HPLC system
equipped with chemiluminescence and UV/VIS detectors. The main component of
the decomposition of peroxide was confirmed to be carbonyl compounds based
on the results of the hydroxylamine hydrochloride titration, IR,C-13-NMR a
nd LC-MS analyses. The formation rate equation of peroxide is derived from
the chain reaction model of lipid radical and the experimental results were
analyzed using the rate equation. The formation rate of the peroxide is in
dependent on the peroxide concentration in the induction period, and is exp
ressed by the first order for the peroxide concentration in the accelerated
period.