Hybrid organic-inorganic particle technology: Breaking through traditionalbarriers of HPLC separations

Hybrid organic-inorganic particles combine the best properties of silica hi gh efficiency and excellent mechanical strength - with the best properties of organic polymers - a wide pH stability range and reduced silanol effects . In this article, the authors describe reversed-phase HPLC packings based on hybrid particles and demonstrate the packing's retention and selectivity characteristics. They also show that these media have long lifetimes at el evated temperatures under conditions at which conventional bonded silica fa ils rapidly. The benefits of operating at elevated temperatures are increas ed efficiency and reduced back pressure. When combined with short columns c ontaining 2.5-mum hybrid particles, these conditions enable fast, high-reso lution gradient separations, which are useful for high-throughput analyses.