Sg. Edwards et B. Seddon, Selective medium based on tyrosine metabolism for the isolation and enumeration of Brevibacillus brevis (Bacillus brevis), LETT APPL M, 31(5), 2000, pp. 395-399
Aims: To develop a selective medium for the enumeration of Brevibacillus br
evis Nagano spores from soil and plant material.
Methods and Results: Tyrosine agar was developed as a selective medium and
compared with nutrient agar for the enumeration of B. brevis Nagano spores
from sterile and non-sterile plant and soil extracts. Brevibacillus brevis
Nagano colonies could be easily identified only on tyrosine agar due to the
ir clear halo and distinct colony morphology. Identification was confirmed
by thin layer chromatography of the antibiotic, gramicidin S, produced by t
his strain.
Conclusions: Tyrosine agar was shown to be a suitable selective medium for
the enumeration of B. brevis Nagano.
Significance and impact of the study: The medium developed, tyrosine agar,
can be used to monitor the population of the biological control agent, B. b
revis Nagano, and will allow detailed studies within the crop environment.