Dynamic cell cycle kinetics in vitro and in vivo in myelodysplastic syndromes with special reference to the influence of hematopoietic growth factors

We have investigated the effect of HGF in vivo and in vitro in MDS using a recently developed FCM assay involving the simultaneous measurement of cell surface antigene, DNA content, and BrdUrd or IodUrd incorporation. This al lows for the determination of the dynamic cell kinetic parameters: LI, T-s, and T-pot and we observed that in vitro HGF stimulation resulted in a sign ificant decrease in mean T-pot values from 6.6 to 3.5 days. Importantly, we demonstrated that in vivo GM-CSF administration to patients with RAEB resu lted in a shortening of T-pot within the 2 first weeks of GM-CSF treatment. (C) 2000 Elsevier Science Ltd. All rights reserved.