The homeodomain protein PRH influences the differentiation of haematopoietic cells

Citation
Ps. Jayaraman et al., The homeodomain protein PRH influences the differentiation of haematopoietic cells, LEUK RES, 24(12), 2000, pp. 1023-1031
Citations number
47
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
LEUKEMIA RESEARCH
ISSN journal
01452126 → ACNP
Volume
24
Issue
12
Year of publication
2000
Pages
1023 - 1031
Database
ISI
SICI code
0145-2126(200012)24:12<1023:THPPIT>2.0.ZU;2-W
Abstract
Haematopoiesis involves the differentiation of a self-renewing stem cell in to all of the lineages found in circulating blood. Myb-Ets transformed chic ken blastoderm cells (MEPs) have many of the characteristics of multipotent haematopoietic cells and represent a useful model system for the study of haematopoiesis. The proline-rich homeodomain protein (PRH) has previously b een shown to be expressed in the haematopoietic compartment. In this report we show that PRH mRNA and protein levels are down regulated as MEPs differ entiate along the myelomonocytic and erythrocytic lineages. In contrast, PR H mRNA and protein levels remain high as MEPs differentiate toward the thro mbocytic lineage. Over-expression of full length PRH in MEPs inhibits their transformation and/or proliferation. However, the over-expression of N-ter minally truncated PRH proteins results in normally proliferating cells that are predominantly differentiated along the myelomonocytic and eosinophilic lineages. These results suggest that PRH plays a role in the proliferation and differentiation of haematopoietic cells. (C) 2000 Elsevier Science Ltd . All rights reserved.