Effect of pH, carbon source and K+ on the Na+-inhibited germ tube formation of Candida albicans

The effect of pH, carbon source and K+ on the Na+-inhibited germ tube forma tion of the pathogenic fungus Candida albicans was examined in the arginine -phosphate modified (APM) medium. All C. albicans cells formed germ tubes i n APM medium at pH 5.0-9.0. Na+ inhibited germ tube formation in a concentr ation dependent manner ranging from 0.2 to 1.0 M, and was further influence d by the pH of the medium. The inhibitory effect of Na+ was lowest at pH 8. 0, and germ tube formation ceased at 1.0 M Na+ for any pH (4.0-9.0). At pH greater than or equal to 6.0, non-germ tube-forming cells did not show yeas t growth; whereas at pH greater than or equal to 5.0, Na+ inhibited only ge rm tube formation but did not inhibit yeast growth. The inhibitory effect o f Na+ was stronger in glucose medium than in galactose medium as carbon sou rce. K+, at 0-0.8 M, had almost no effect on germ tube formation. However, in the presence of Na+, a very low concentration of K+ (0.5 mM) was able to release the cells from Na+ arrest and produced an increase in the rate as well as the percentage of germ tube formation. Intracellular Na+/K+ ratios increased with the increase in extracellular Na+ concentration, whereas the ratios decreased and remained within nontoxic levels when the extracellula r K+ concentration was increased.