A simplified method for sample collection and DNA isolation for polymerasechain reaction detection of Trypanosoma rangeli and Trypanosoma cruzi in triatomine vectors
Emm. Machado et al., A simplified method for sample collection and DNA isolation for polymerasechain reaction detection of Trypanosoma rangeli and Trypanosoma cruzi in triatomine vectors, MEM I OSW C, 95(6), 2000, pp. 863-866
Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in
Central and South America, sharing several reservoirs and triatomine vector
s, we herein describe a simple method to collect triatomine feces and hemol
ymph in filter paper for further detection and specific characterization of
these two trypanosomes. Experimentally, infected triatomines feces and hem
olymph were collected in filter paper and specific detection of T. rangeli
or T. cruzi DNA by polymerase chain reaction was achieved. This simple DNA
collection method allows sample collection in the field and further specifi
c trypanosome detection and characterization in the laboratory.