Effect of peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum - Effect of antibiotics on RNase P

Authors
Stathopoulos, C Tsagla, A Tekos, A Drainas, D
Citation
C. Stathopoulos et al., Effect of peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum - Effect of antibiotics on RNase P, MOL BIOL RP, 27(2), 2000, pp. 107-111
Citations number
32
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY REPORTS
ISSN journal
03014851 → ACNP
Volume
27
Issue
2
Year of publication
2000
Pages
107 - 111
Database
ISI
SICI code
0301-4851(200006)27:2<107:EOPIOR>2.0.ZU;2-0
Abstract
The effect of several peptidyltransferase inhibitors on ribonuclease P acti vity from Dictyostelium discoideum was investigated. Among the inhibitors t ested puromycin, amicetin and blasticidin S revealed a dose-dependent inhib ition of tRNA maturation. Blasticidin S and amicetin do not compete with pu romycin for the same site on the enzyme, suggesting the existence of distin ct antibiotic binding sites on D. discoideum RNase P. Inhibition experiment s further indicate that binding sites for blasticidin S and amicetin overla p.