Optimal fabrication condition of bacteriorhodopsin thin films onto modified self-assembled monolayers

The optimal condition of bacteriorhodopsin (bR) adsorption onto the modifie d self-assembled monolayers (SAMs) was investigated. The SAMs of 11-mercapt oundecanoic acid (11-MUA) on the pre-treated gold substrate were used as a template, and modified with poly-l-lysine (PL) For the effective adsorption of bR. Based on the photoelectric responses of the bR thin films, it could be found that the optimal bR suspension concentration and buffer pH for bR thin film fabrication were determined as 5 mg/mL and pH 8, respectively.