Capillary oxygen saturation and tissue oxygen pressure in the rat cortex at different stages of hypoxic hypoxia

The objective of this study was to generate data that allow for estimation of the validity of oxygen saturation (SO2)values in superficial cortical ca pillaries as calculated by a microreflectometric system (EMPHO II(R)). Capi llary SO2 and tissue oxygen pressure (PtO2 were measured simultaneously in the cortex of n = 13 Wistar rats under normocapnic (PaCO2 = 36 mmHg) arteri al normoxia (PaO2 = 92 mmHg), moderate (paO(2) = 53 mmHg) and severe hypoxi c hypoxia (PaO2 = 31 mmHg) with microreflectometry and multiwire surface el ectrodes. Values were pooled according to arterial oxygenation levels, disp layed as frequency histograms and compared via ANOVA (p < 0.05). In a Hill- plot (log PtO2 versus log SO2/100-SO2)) an in vivo tissue oxygen dissociati on curve was obtained and a linear regression/correlation analysis performe d Mean<plus/minus>SD values of SO2 respectively PtO2 decreased from 45.6% /- 14.6% resp. 26.8 +/- 8.2 mmHg during arterial normoxia to 32.6% +/- 10.2 % resp. 20.2 +/- 6.6 mmHg during moderate and to 12.3% +/- 11.1% resp. 8.7 +/- 5.0 mmHg during severe hypoxic hypoxia. Linear regression analysis in t he Hill-plot of values between 1% and 65% SO2 and 0.1 and 41 mmHg PtO2 reve aled an excellent correlation (r(2) = 0.88) with an increase of scatter bel ow 10% SO2 or 1.5 mmHg PtO2. We conclude that SO2 values calculated by the algorithm of the applied microreflectometric system reflect very accurately cortical oxygen supply over a very wide range of oxygenation levels when c ompared to a gold standard reference. Only at extremely low levels (e.g bel ow 10% SO2) did we iind possible inaccuracies with regard to truly absolute saturation values.