DNA-triplex stabilizing properties of 8-aminoguanine

A DNA-triplex stabilizing purine (8-aminoguanine) is designed based on mole cular modeling and synthesized, The substitution of guanine by 8-aminoguani ne largely stabilizes the tripler both at neutral and acidic pH, as suggest ed by molecular dynamics and thermodynamic integration calculations, and de monstrated by melting experiments, NMR experiments confirm the tripler-stab ilizing properties of 8-aminoguanine and demonstrate that few changes are f ound in the structure of the tripler due to the presence of this modified b ase.