Localization of a nod factor-binding protein in legume roots and factors influencing its distribution and expression

Citation
G. Kalsi et Me. Etzler, Localization of a nod factor-binding protein in legume roots and factors influencing its distribution and expression, PLANT PHYSL, 124(3), 2000, pp. 1039-1048
Citations number
62
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT PHYSIOLOGY
ISSN journal
00320889 → ACNP
Volume
124
Issue
3
Year of publication
2000
Pages
1039 - 1048
Database
ISI
SICI code
0032-0889(200011)124:3<1039:LOANFP>2.0.ZU;2-T
Abstract
The roots of the legume Dolichos biflorus contain a lectin/nucleotide phosp hohydrolase (Db-LNP) that binds to the Nod factor signals produced by rhizo bia that nodulate this plant. In this study we show that Db-LNP is differen tially distributed along the surface of the root axis in a pattern that cor relates with the zone of nodulation of the root. Db-LNP is present on the s urface of young and emerging root hairs and redistributes to the tips of th e root hairs in response to treatment of the roots with a rhizobial symbion t or with a carbohydrate ligand. This redistribution does not occur in resp onse to a non-symbiotic rhizobial strain or a root pathogen. Db-LNP is also present in the root pericycle where its level decreases upon initiation of nodule formation. Maximum levels of Db-LNP are found in 2-d-old roots, and the expression of this root protein is increased when the plants are grown in the absence of NO3- and NH4+. These results support the possibility tha t Db-LNP is involved ii! the initiation of the Rhizobium legume symbiosis.