Stepwise solid-phase synthesis of peptide-oligonucleotide phosphorothioateconjugates employing Fmoc peptide chemistry

Authors
Antopolsky, M Azhayev, A
Citation
M. Antopolsky et A. Azhayev, Stepwise solid-phase synthesis of peptide-oligonucleotide phosphorothioateconjugates employing Fmoc peptide chemistry, TETRAHEDR L, 41(47), 2000, pp. 9113-9117
Citations number
4
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
41
Issue
47
Year of publication
2000
Pages
9113 - 9117
Database
ISI
SICI code
0040-4039(20001118)41:47<9113:SSSOPP>2.0.ZU;2-L
Abstract
A straightforward stepwise method for the preparation of peptide-oligonucle otide phosphorothioate conjugates, was developed, based on the highly effic ient Fmoc peptide solid phase synthesis, followed by oligonucleotide phosph orothiate chain assembly. The three conjugates synthesized contained 15- or 17-mer oligonucleotide phosphorothioates and 10- or 16-mer peptides, incor porating two or three arginine residues. (C) 2000 Elsevier Science Ltd. All rights reserved.