TROSY and CRINEPT: NMR with large molecular and supramolecular structures in solution

TROSY and CRINEPT are new techniques for solution NMR studies of molecular and supramolecular structures. They allow the collection of high-resolution spectra of structures with molecular weights >100 kDa, significantly exten ding the range of macromolecular systems that can be studied by NMR in solu tion. TROSY has already been used to map protein-protein interfaces, to con duct structural studies on membrane proteins and to study nucleic acid conf ormations in multimolecular assemblies. These techniques will help us to in vestigate the conformational states of individual macromolecular components and will support de novo protein structure determination in large supramol ecular structures.