The effect of fat rich diet and alcohol on ion concentration in bile fluidin rats

The metabolic alteration of fatty liver causes significant changes in the c omposition of bile fluid secreted by epithelial liver cells. These processe s include both organic molecular and inorganic ion content changes in bile juice as well. In this work ion concentration differences of bile fluid in experimental hyperlipidemic rats compared to normal ones were monitored. Fa tty liver in young male Wistar albino rats was induced by 2% cholesterol, 2 0% sunflower oil and 0.5% cholic acid added to the normal food and 3% alcoh olic water was administered to rats for 9 days. The development of hyperlip idemy was detected by measuring serum parameters and fatty liver was proved by morphological investigations. The ion concentrations of bile fluid samp les were determined by ICP-OES (inductively coupled plasma optical emission spectrometry). The bile fluid samples mere digested with a mixture of HNO3 and H2O2. The results show that the concentration of calcium and phosphoru s in bile juice increased significantly in hyperlipidemic rats compared to the control. Significant concentration changes have also been detected for chromium and manganese. The changes in metal ion metabolism also point to t he damage of liver cells owing to metal complex evacuation.