Crystallization of an AMPA receptor binding domain without agonist: importance of carbohydrate content and flash-cooling conditions

An AMPA-specific ionotropic glutamate receptor binding domain was overexpre ssed using the baculovirus system and purified by immunoaffinity and metal- affinity chromatography. Purified protein was enzymatically deglycosylated. Both glycosylated and deglycosylated proteins crystallized under the same conditions and in the same space group (P2). In both cases, it was observed that the use of MPD as a cryoprotectant induced a significant reduction in the unit-cell volume compared with glycerol or sucrose. For crystals of de glycosylated protein, cryoprotection with MPD also yielded a dramatic impro vement in resolution.