Crystallization and preliminary X-ray analysis of earthworm fibrinolytic enzyme component A from Eisenia fetida

Earthworm fibrinolytic enzyme component A, a protein which functions both a s a direct fibrinolytic enzyme and a plasminogen activator, was purified fr om the earthworm Eisenia fetida. Diffraction-quality single crystals of the protein were grown by the hanging-drop vapour-diffusion technique with amm onium sulfate as a precipitant. The crystals belong to the orthorhombic spa ce group P2(1)2(1)2(1), with unit-cell parameters a = 40.6, b = 127.5, c = 129.2 Angstrom and three molecules per asymmetric unit. The data set reache d a resolution of 1.95 Angstrom.