Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis

The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs fro m the previously published protein sequence, which was used for the origina l structure determination, at 16 positions. The differences include three a dditional inserted residues, one deleted residue and 12 point substitutions . The gene was subcloned and the recombinant protein overexpressed, purifie d and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 Angstrom resolution.