Cj. Bond et al., Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis, ACT CRYST D, 56, 2000, pp. 1359-1366
The gene encoding the iron-dependent superoxide dismutase from Pseudomonas
ovalis was cloned from a genomic library and sequenced. The ORF differs fro
m the previously published protein sequence, which was used for the origina
l structure determination, at 16 positions. The differences include three a
dditional inserted residues, one deleted residue and 12 point substitutions
. The gene was subcloned and the recombinant protein overexpressed, purifie
d and crystallized in a trigonal space group. The structure was determined
by molecular replacement and was refined to 2.1 Angstrom resolution.