Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Bacillus megaterium IWG3

Authors
Yamamoto, K Kusunoki, M Urabe, I Tabata, S Osaki, S
Citation
K. Yamamoto et al., Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Bacillus megaterium IWG3, ACT CRYST D, 56, 2000, pp. 1443-1445
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
11
Pages
1443 - 1445
Database
ISI
SICI code
0907-4449(200011)56:<1443:CAPXAO>2.0.ZU;2-E
Abstract
Glucose dehydrogenase from Bacillus megaterium IWG3 has been crystallized i n the presence of NAD(+) using the hanging-drop vapour-diffusion method wit h PEG 2000 as the precipitant. Crystals belong to space group C2 and have u nit-cell parameters a = 120.8 (1), b = 66.7 (1), c = 119.6 (1) Angstrom, be ta = 93.25 (3)degrees with standard deviations in parentheses. Assumption o f four subunits in the asymmetric unit gave the most probable Matthews coef ficient V-M of 2.1 Angstrom (3) Da(-1) (solvent content 41.7% by volume). X -ray diffraction data were collected to 1.7 Angstrom on a synchrotron-radia tion source.