Purification, crystallization and preliminary diffraction study of beta-galactosidase from Penicillium sp.

Authors
Neustroev, KN de Sousa, EA Golubev, AM Neto, JRB Eneyskaya, EV Kulminskaya, AA Polikarpov, I
Citation
Kn. Neustroev et al., Purification, crystallization and preliminary diffraction study of beta-galactosidase from Penicillium sp., ACT CRYST D, 56, 2000, pp. 1508-1509
Citations number
17
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
11
Pages
1508 - 1509
Database
ISI
SICI code
0907-4449(200011)56:<1508:PCAPDS>2.0.ZU;2-P
Abstract
Crystals of an extracellular beta -galactosidase from Penicillium sp. (MW = 120 +/- 5 kDa) have been obtained from a sodium phosphate buffer using PEG as precipitant. The crystals belong to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 110.82, c = 161.28 Angstrom, and diffract to 1.85 Angstrom resolution at a synchrotron source.