Protecting water quality and maintaining profitable corn (Zea mays L.) prod
uction requires diagnostics that can distinguish between N deficieny, suffi
ciency, and excess. This study evaluates initial recommendations on blackla
yer basal-stalk NO3-N ranges and critical concentrations for diagnosing N s
tatus, and it compares the performance of this test with gain analysis, Obs
ervations (428) were collected from 13 N-response experiments. linear respo
nse and plateau (LRP) and binary logistic regression (BLR) were used to cha
racterize the relationships between yield and tissue-test values. With the
LRP, stalk NO3-N and grain N concentrations separating deficient front suff
icient observations were 0.42 and 13.1 kg(-1), respectively; and the succes
s rates of the two tests were comparable (77 and 75%, respectfully). The BL
R also identified critical concentrations, but the values increased with de
creasing yields, a desirable decision-rule attribute given that extreme def
iciency can result in higher-than-expected tissue concentrations. The succe
ss rates of multiple BLR functions using yield and stalk or grain analysis
as factors were again comparable (88 and 87%, respectively), but they were
significantly greater than with the LRP analysis. Stalk analysis was superi
or to grain analysis for distinguishing sufficiency from excess. Ii constan
t stalk NO3-N concentration (1.67 g kg(-1)) separated sufficient from exces
sive cases, and fertilizer efficiency approached zero at 2.9 g kg(-1). Prem
ature sampling resulted in stalk. NO3-N levels that were 40 to 600% greater
than levels observed after blacklayer formation, with the greatest error o
ccurring when N fertility was low. When not testing for N excess, the advan
tages of grain analysis are the ease of sampling during harvest and the red
uced risk of error associated with premature sampling.