Increased activity and intranuclear expression of phospholipase D2 in human renal cancer

We examined the PLD activities of human renal cancers and found that the PL D2 activity was greatly elevated in almost all cases examined as compared w ith the adjacent normal region. Western blot analysis showed the increased levels of PLD2 protein, but the PLD1 was not discernible. The oleate-depend ent PLD activity was very low but appeared to increase in most cases. Inter estingly, the immunohistochemical observations indicated the high expressio n of PLD2 in the nuclei of clear carcinoma cells. This is the first demonst ration which suggests the possible involvement of PLDS in tumorigenesis of renal cancer. (C) 2000 Academic Press.