Umbilical cord blood transplantation from unrelated HLA-matched donor in an adult with severe aplastic anemia

A 23-year-old male suffering from severe aplastic anemia (SAA) weighing 60 kg was successfully treated by unrelated allo-CBSCT (cord blood stem cell t ransplantation). A six-loci HLA-identical umbilical cord blood (UCB) was in fused after conditioning with low-dose cyclophosphamide (CTX) and antilymph ocyte globulin (ALG), The prophylaxis of GVHD consisted of CsA and MTX, The infused cord blood provided 1.89 x 10(7) nucleated cells per kg, CD34-posi tive cells: 0.89%, Neutrophils >0.5 x 10(9)/l were reached 10 days after tr ansplant, and platelets greater than 50.0 x 10(9)/l at day 26, RBC and plat elet transfusion independence were reached on days 15 and 18, The patient d eveloped grade 1 skin GVHD 10 months after engraftment of the donor cells, Microsatellite DNA fingerprinting indicated a stable and persistent donor-r ecipient mixed chimerism, whilst the circulating red cells remain of host o rigin.