Retention behavior of reversed-phase for HPLC using a 100% aqueous mobile phase

Authors
Citation
N. Nagae et T. Enami, Retention behavior of reversed-phase for HPLC using a 100% aqueous mobile phase, BUNSEKI KAG, 49(11), 2000, pp. 887-893
Citations number
10
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
BUNSEKI KAGAKU
ISSN journal
05251931 → ACNP
Volume
49
Issue
11
Year of publication
2000
Pages
887 - 893
Database
ISI
SICI code
0525-1931(200011)49:11<887:RBORFH>2.0.ZU;2-X
Abstract
The retention behavior of the reversed-phase was evaluated under 100% aqueo us conditions. It is commonly said that reversed-phases, such as ODS (C-18) and C-8, show a decrease in the retention time under 100% aqueous conditio ns. The mechanism of the decrease in retention was found to be that the 100 % aqueous mobile phase was pulled out of the pore of the packing materials, so that the stationary phase in contact with the mobile phase decreased. S ome parameters, such as the pore size, length of the alkyl group of the sta tionary phase, temperature and buffer in the mobile phase, were shown to in fluence the decrease in retention. The C-30 phase didn't show any decrease of retention. Furthermore, ODS, which had a larger pore, didn't show any de crease in the retention time, but showed high reproducibility in retention. In contrast, TMS (C-1), which had a smaller pore, showed a decrease of ret ention, although it was known that TMS, which had 10 nm of a pore, didn't s how a decrease in retention. The reversed-phase could be used under 100% aq ueous conditions, and showed high reproducibility in retention when it was filled with some terms.